PL inactivation levels for tiffany earrings were significantly higher than in cider, juice, or TS (Figs. 2 and 3). In the case of PL treatment of P at fluence levels higher than 2.0 J/cm^sup 2^, the MPN technique was used to estimate the numer of survivors. tiffany key rings dotted line in Figure 2 represents the oundary etween the data otained using the standard plate counting technique and the data otained y the MPN technique.These results suggest that the level of inactivation was limited mostly y the asorption of light in the UV range. oth apple juice tiffany keys cider showed high asorance of light in the range of wavelengths from 200 to 400 nm, while TS asors smaller amounts of light at wavelengths of >300 nm, which leads to higher inactivation levels of E. coli ATCC 25922 in this sustrate than in juice and cider. These oservations are consistent with the findings of Koutchma and Parisi (10), who found that the tiffany money clips asorance of different caramel model solutions of apple juice and cider affected the efficiency of continuous UV light treatment. Validation of E. coli ATCC 25922 as a surrogate organism for E. coli O157:H7. The efficacy of any antimicroial technology is typically tested in the laoratory y using the most relevant pathogens for a particular application as challenge organisms. efore its commercial application, the efficacy of the process must e validated using surrogate microorganisms. To date, no surrogate identification and validation has een reported for the pathogenic E. coli O157:H7 treated with PL.
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