lfm1001

Survivors were counted using the standard plate counting method. Tiffany Knots ring plate counts fell elow 25 CFU, estimation of survivors was performed using the most-proale-numer technique (MPN) (20). The treated liquid (10 ml) was transferred into 100 ml of Paloma's Tenderness Heart ring in the following distriution: 3 tues with 10 ml, 3 tues with 1 ml, and 3 tues with 0.1 ml. The TS tues then were incuated at 35 2 C for 48 2 h. Turidity was used to presumptively identify positive samples. The inoculum and survivor counts were expressed in log CFU and log MPN, respectively. All standard plate counting and Tiffany Notes band ring analyses were performed in duplicate. The level of microial reduction was calculated as log), and inactivation curves were uilt y plotting reduction against fluence (F).Sustrate characterization. As light asorption y the sustrate diminishes the amount of light availale to the microial cells, Tiffany Notes ring was important to quantify the asorption properties of the treated sustrates. The asorance spectra of the four liquid sustrates were measured, and the results are shown in Figure 1. Since the UV range is the portion of the road-spectrum light that is critical for microial inactivation (25), asorption in the UV range is the most important for the outcome of PL treatment. While P did not show significant asorance of UV light, the three other sustrates asored fairly high amounts of light in this range. oth apple juice and apple cider showed high asorance around 浣?of 254 nm, the region that is often credited as having the maximum actericidal effect .